human umbilical vein ndothelial cells huvecs  (ATCC)

Journal: Journal of the American College of Cardiology

Article Title: The tyrosine phosphatase, SHP-1, is a negative regulator of endothelial superoxide formation.

doi: 10.1016/j.jacc.2005.02.039

Figure Lengend Snippet: Figure 1. SH2-domain containing tyrosine phosphatase-1 (SHP-1) is constitutively active in endothelial cells and degraded by antisense- magnetofection. Magnetofection of antisense oligodesoxynucleotide (AS- ODN) against SHP-1 into human umbilical vein endothelial cells (HUVEC) resulted in deprivation of its protein associated with a loss of SHP-1 activity. Note that there is remarkable activity of SHP-1 in HUVEC grown under control conditions (random ODN, similar activity observed in untransfected cells, see Fig. 4). **Significantly different vs. control at p 0.01.

Article Snippet: Primary human umbilical vein ndothelial cells (HUVEC) were harvested from umbilical ords by digestion with dispase (2.4 U/ml) and grown to onfluence in medium 199 supplemented with 20% neworn calf serum and 20% endothelial growth medium Promocell, Heidelberg, Germany) as previously described HUVEC medium) (21). agnetofection of oligonucleotides.

Techniques: Magnetofection, Activity Assay, Control

Journal: Journal of the American College of Cardiology

Article Title: The tyrosine phosphatase, SHP-1, is a negative regulator of endothelial superoxide formation.

doi: 10.1016/j.jacc.2005.02.039

Figure Lengend Snippet: Figure 5. SH2-domain containing tyrosine phosphatase-1 (SHP-1) down- regulates reduced nicotinamide adenine dinucleotide (phosphate) (NAD[P]H)-oxidase activity. (A) Specific inhibitory peptides against NAD(P)H-oxidase (gp91ds-tat, 100 mol/l) abrogated the increase in O2 · release in human umbilical vein endothelial cell (HUVEC) mono- layers that was caused by sodium stibogluconate (SS). (B) NADH- dependent O2 · production in HUVEC protein lysates was increased 3.3-fold after treatment of cells with antisense oligodesoxynucleotide (AS-ODN) against SHP-1 but not with random ODN. *, **Significantly different vs. control at p 0.05 and p 0.01, respectively; ##significantly different vs. SS at p 0.01.

Article Snippet: Primary human umbilical vein ndothelial cells (HUVEC) were harvested from umbilical ords by digestion with dispase (2.4 U/ml) and grown to onfluence in medium 199 supplemented with 20% neworn calf serum and 20% endothelial growth medium Promocell, Heidelberg, Germany) as previously described HUVEC medium) (21). agnetofection of oligonucleotides.

Techniques: Activity Assay, Control

Journal: Journal of the American College of Cardiology

Article Title: The tyrosine phosphatase, SHP-1, is a negative regulator of endothelial superoxide formation.

doi: 10.1016/j.jacc.2005.02.039

Figure Lengend Snippet: Figure 4. Influence of vascular endothelial growth factor (VEGF) on SH2-domain containing tyrosine phosphatase-1 (SHP-1) activity and superoxide production. (A) Stimulation with VEGF (10 ng/ml) enhanced basal phosphatase activity of SHP-1 in human umbilical vein endothelial cells (HUVEC). (B) VEGF (10 ng/ml) also increased HUVEC O2 ·

Article Snippet: Primary human umbilical vein ndothelial cells (HUVEC) were harvested from umbilical ords by digestion with dispase (2.4 U/ml) and grown to onfluence in medium 199 supplemented with 20% neworn calf serum and 20% endothelial growth medium Promocell, Heidelberg, Germany) as previously described HUVEC medium) (21). agnetofection of oligonucleotides.

Techniques: Activity Assay